Reporter

Part:BBa_K784027:Design

Designed by: Lior Levy   Group: iGEM12_Technion   (2012-09-17)


XhoI+RBS+Alkaline Phosphatase (phoA) +XmaI


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1489
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1489
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1489
    Illegal XhoI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1489
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1489
    Illegal NgoMIV site found at 328
    Illegal NgoMIV site found at 775
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

we made XhoI and XmaI sites on both sides in order to make ligation with this part


Source

the RBS and phoA part are from the parts registry

References